FIELD: biotechnologies.
SUBSTANCE: invention proposes a method for obtaining immobilised biocatalyst for synthesis of water solutions of amides, including acrylamide and nicotinamide from nitriles of carboxylic acids. Chitosan microgranules with diameter of 1-2 mm are obtained as a result of forcing-through of its 2-4% solution in 2% acetic acid by means of an extrusion machine to 1M of potassium hydroxide solution. Then, chitosan is activated with 0.01-0.5% solution of benzoquinone in the ratio of 1:1. Then, ferment preparation of nitrile hydrase is immobilised at 20 minutes of incubation at the temperature of 22-25°C.
EFFECT: immobilised nitrile hydrase at conversion of nitriles keeps its activity at least in fifty cycles of reactions.
5 dwg, 5 ex
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