FIELD: medicine.
SUBSTANCE: Atragene speciosa Weinm. (Siberian clematis) plant cells are cultivated on a MC medium illuminated by white light of intensity (2-4)×103 lux with photoperiod 12-14 hour at the concentration of 2,4-dichlorphenoxyacetic acid 0.6-1.2 mg/l, 6-benzylaminopurine 0.2-0.6 mg/l, mesoinositol 100-125 mg/l. Additional introduction of phytohormone 24-epibrassinolide to the nutrient medium increases the cell percentage of an end product.
EFFECT: method allows producing a required volume of callus culture biomass with high content of triterpene saponosides and flavonoids close to such in leaves of an intact plant.
2 cl, 2 tbl, 3 ex
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