METHOD OF OBTAINING MYCOPLASMOSIS DIAGNOSTIC SERUMS FOR INDIRECT IMMUNOFLUORESCENCE REACTION BY RABBIT HYPERIMMUNISATION Russian patent published in 2009 - IPC A61K39/395 C12P21/00 G01N33/569 C12R1/35 

FIELD: medicine.

SUBSTANCE: invention relates to obtaining mycoplasmosis diagnostic serums for diagnosis of mycoplasmoses in pigs in indirect immunofluorescence reaction. Method includes use of antigens from three mycoplasma cultures - Mycoplasma hyosynoviae, M. hyorhinis and Ureaplasma sp.B which are used for rabbit hyperimmunisation during 21 days by divided intravenous antigen introduction every three days with triple application of immunostimulator levomisol. Antibody synthesis dynamics is studied on 7, 14 and 21 day after first antigen introduction in indirect immunofluorescence reaction (IIFR). Reaction is carried out according to conventional methodology. Four-cross system is used for reaction estimation. It has been established that on the 7-th day after first antigen introduction antibodies in IIFR were detected in low titers 1:10-1:20. On the 14-th day positive reaction in IIFR was at higher dilutions within 1:320-1:640, and on the 21-st day antibody synthesis reached maximal level 1:640-1:1280.

EFFECT: obtaining anti-mycoplasmosis serums in higher diagnostic antibody titers, reduction of hyperimmunisation terms and possibility to use obtained serums for diagnosis of mycoplasmoses in pigs in IIFR.

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