METHOD OF PRODUCING ANTICOAGULATIVE PROTEIN, EXTRACTED FROM NEMATODES (NAP) Russian patent published in 2009 - IPC C12P21/02 C07K14/435 A61K38/17

Abstract RU 2355770 C2

FIELD: biology.

SUBSTANCE: present invention relates to genetic engineering, more specifically to obtaining anticoagulative protein extracted from nematodes (NAP) and can be used in medicine. To obtain a medicinal preparation based on NAP, methanotrophic yeast host cells are cultured, encoding rNAPc2 or rNAPc2/praline, until attaining the desired cell density. NAP is then extracted from the said yeast host cells through cation-exchange chromatography on an expanding layer. To purify the NAP medicinal preparation, hydrophobic-interaction chromatography is used. NAP is extracted and purified at pH levels below 4.

EFFECT: simple and more efficient method of obtaining anticoagulation proteins from nematodes.

25 cl, 8 dwg, 7 tbl, 6 ex

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RU 2 355 770 C2

Authors

Mendoza Kristin B.

Liddell Dzhon

Moss Dehvid

Dates

2009-05-20—Published

2004-05-14—Filed