FERMENTATION MEDIUM AND METHOD OF PRODUCTION OF RECOMBINANT PROTEINS Russian patent published in 2013 - IPC C12P1/02 C12P1/04 C12N1/14 C12N1/20 C12N9/00 

FIELD: biotechnology.

SUBSTANCE: fermentation medium for production of recombinant proteins using methanol inducible fungi of species Pichia pastoris is characterised by maintained concentration of urea or its derivatives in the range from about 0.3 M to about 1 M. The medium contains per liter of water basic salts in the following amounts: orthophosphoric acid (85%) from 2.67 to 133.5 ml, calcium sulfate from 0.093 to 4.65 g, potassium sulfate from 1.82 to 91 g, magnesium sulfate-7H₂O from 1.49 to 74.5 g, potassium hydroxide from 0.413 to 20.65 g, glycerol from 4 to 200 g, and microelements per liter of water in the following amounts: copper sulfate-5H₂O from 0.6 to 30 g, sodium iodide from 0.008 to 0.4 g, manganese sulfate-H₂O from 0.3 to 15 g, sodium molybdate-H₂O from 0.02 to 1 g, boric acid from 0.002 to 0.1 g, cobalt chloride from 0.05 to 2.5 g, zinc chloride from 2 to 100 g, sulphate divalent iron-7H₂O from 6.5 to 325 g, biotin from 0.02 to 1 g, sulfuric acid from 0.5 to 25 ml. The method for production of recombinant proteins comprises the stage of reproduction of methanol inducible fungi of species Pichia pastoris, and the stage of recombinant protein expression using the said fermentation medium in which feeding with methanol is carried out at a rate of about 6 g/l/hr to about 20 g/l/hr.

EFFECT: increased yield of target products.

11 cl, 27 dwg, 16 ex