FIELD: biotechnology.
SUBSTANCE: invention relates to microbiology, genetic engineering, biotechnology, molecular biology. Group of inventions discloses a recombinant Pichia pastoris strain in which a synthetic gene with codons adapted for P. pastoris, which codes human alpha-fetoprotein (AFP), is inserted. What is also described is a method for preparing the AFP and a pharmaceutical composition which can be used in anticancer therapy.
EFFECT: output of AFP is up to 300 mg/l, AFP purity is 95%.
4 cl, 1 dwg, 11 ex
| Title | Year | Author | Number |
|---|---|---|---|
| CONJUGATE OF ANTITUMOUR DRUGS WITH RECOMBINANT ALPHA-FETOPROTEIN AND ITS FUNCTIONAL FRAGMENTS AND METHOD OF THEIR PRODUCING | 2016 |
|
RU2630974C1 |
| METHOD FOR PRODUCING ACTIVE FRAGMENT OF HUMAN ALPHA-FETOPROTEIN | 2010 |
|
RU2448116C2 |
| ANTITUMOR PEPTIDE PREPARATION BASED ON ALPHA-FETOPROTEIN FRAGMENT, ITS CONJUGATE, PHARMACEUTICAL COMPOSITION AND METHOD FOR TREATMENT OF HORMONE-DEPENDENT TUMORS | 2005 |
|
RU2285537C1 |
| Escherichia coli BL21(DE3)/pAFP11D3 STRAIN - PRODUCER OF 404 TO 609 AMINO ACID FRAGMENT OF HUMAN ALPHA-FETOPROTEIN | 2010 |
|
RU2422512C1 |
| STRAIN OF YEAST PICHIA PASTORIS AS PRODUCER OF LEUKOCYTE HUMAN INTERFERON-16, RECOMBINANT PLASMID DNA PHIN AND METHOD FOR ITS CONSTRUCTION | 2002 |
|
RU2203950C1 |
| YEAST Pichia pastoris X-33/2albumin STRAIN FOR SECRETION OF HUMAN ALBUMIN | 2005 |
|
RU2306333C2 |
| RECOMBINANT PLASMID FOR PHYTASE GENE EXPRESSION IN PICHIA PASTORIS YEAST (VERSIONS), PICHIA PASTORIS YEAST STRAIN -PHYTASE PRODUCER (VERSIONS) | 2009 |
|
RU2409670C1 |
| INTEGRATIVE PLASMID VECTOR FOR EXPRESSION OF GENES IN YEAST | 2008 |
|
RU2388823C1 |
| RECOMBINANT DNA FRAGMENT, ENCODING HUMAN ALPHA-FETOPROTEIN (AFP), CONTAINING "SLOW" LEUCINE-ENCODING CODONS, EXPRESSION PLASMID, CONTAINING SAID FRAGMENT, SACCHAROMYCES CEREVISIAE CELL, TRANSFORMED WITH SAID PLASMID, AND METHOD FOR OBTAINING HUMAN AFP | 2015 |
|
RU2617943C2 |
| PICHIA PASTORIS 2-2 YEAST STRAIN AS PRODUCER OF HUMAN PLATELET GROWTH FACTOR (PDGF-BB) AND METHOD FOR PRODUCTION OF HUMAN PLATELET GROWTH FACTOR | 2005 |
|
RU2290434C1 |
