FIELD: bioengineering.
SUBSTANCE: recombinant strain of fungus Penicillium verruculosum BKM F-4587D, and strain of fungus Penicillium verruculosum BKM F-4586D are suggested, they are producers of the high-active complex of cellulolytic enzymes of the fungus P. verruculosum and heterologous β-glycosidase from A. niger and pectinlyase A from P. canescens. The strain P. verruculosum BKM F-4587D and strain P. verruculosum BKM F-4586D have, respectively, similar or in mainly enzymic activity in relation to the birch xylan and citrus pectin with degree of etherification 70%. The options of production of the enzymatic agent having xylanlytic and pectolytic activity are suggested, the method means cultivation of the strain P. verruculosum BKM F-4587D, or strain P. verruculosum BKM F-4586D. The enzymatic agent based on the strain P. verruculosum BKM F-4587D or on the strain P. verruculosum BKM F-4586D is used respectively under methods of mash production from the chokeberry or grapes.
EFFECT: production of the enzymatic agents with high pectolytic and cellulolytic activity.
6 cl, 3 dwg, 5 tbl, 6 ex
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