FIELD: biotechnology.
SUBSTANCE: disclosed is a recombinant strain Penicillium verruculosum B6/PMO-BG-25, intended for production of homologous lytic polysaccharide monooxygenase, homologous cellobiohydrolase I and heterologous β-glucosidase of Aspergillus niger, as well as a method of producing an enzyme preparation B6/PMO-BG-25 using said recombinant strain.
EFFECT: invention provides a reduction in the cultivation time of said recombinant strain, as well as an increase in the glucose output and reducing sugars during enzymatic hydrolysis of cellulose-containing materials using said enzyme preparation.
2 cl, 2 dwg, 1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| GENETIC FUSION-CONSTRUCTION FOR PROVIDING EXPRESSION OF MULTIENZYMATIC COMPLEX OF CARBOHYDRASES IN CELLS OF MYCELIUM FUNGUS Penicillium verruculosum, APPLIED AS HOST, METHOD OF OBTAINING RECOMBINANT STRAIN OF FUNGUS Penicillium verruculosum AND METHOD OF OBTAINING ENZYMATIC MEDICATION ON ITS BASE | 2013 |
|
RU2550044C2 |
| NEW RECOMBINANT STRAIN (VERSIONS) OF MYCELIAL FUNGUS PENICILLIUM VERRUCULOSUM, AND ENZYMATIC AGENT (VERSIONS) INTENDED FOR HYDROLYSIS OF FRUIT RAW MATERIAL, AND METHOD OF ITS PRODUCTION | 2014 |
|
RU2574206C1 |
| GENETIC CONSTRUCT FOR EXPRESSION OF A COMPLEX OF ENZYMES OF ENDOGLUCANASES AND XYLANASES IN THE CELLS OF THE FUNGUS PENICILLIUM VERRUCULOSUM AND METHOD FOR OBTAINING COMPLEX ENZYME PREPARATIONS BASED ON IT, INTENDED FOR FODDER PRODUCTION | 2017 |
|
RU2653429C1 |
| STRAIN OF MYCELIAL MUSHROOM PENICILLIUM VERRUCULOSUM - PRODUCER OF COMPLEX OF CELLULASE, XYLANASE AND XYLOGLUCANASE AND WAY OF RECEPTION OF FERMENTAL PREPARATION OF COMPLEX OF CELLULASE, XYLANASE AND XYLOGLUCANASE FOR HYDROLYSIS OF CELLULOSE AND HEMICELLULOSE | 2008 |
|
RU2361918C1 |
| STRAIN OF PENICILLIUM VERRUCULOSUM PRODUCER OF PHYTASE AND ENDO-1,4-β-GLUCANASE II COMPLEX AND ENZYME PREPARATION BASED ON IT FOR USE AS ADDITIVE IN FEED | 2022 |
|
RU2810538C2 |
| RECOMBINANT STRAIN OF MYCELIAL FUNGUS PENICILLIUM VERRUCULOSUM (VERSIONS) AND A METHOD OF PRODUCING ENZYME PREPARATION WITH ITS USE (VARIANTS) | 2015 |
|
RU2646136C2 |
| STRAIN OF FUNGUS PENICILLIUM VERRUCULOSUM EE-105, PRODUCER OF A COMPLEX OF HIGHLY EFFECTIVE ENDOGLUCANASES AND AN ENZYME PREPARATION BASED THEREON FOR USE AS A FODDER ADDITIVE IN CEREAL FODDERS | 2018 |
|
RU2696036C1 |
| PENICILLIUM CANESCENS MTCBHI FUNGUS STRAIN PRODUCER OF THERMALLY STABLE CELLOBIOHYDROLASE I AND AN ENZYME PREPARATION BASED THEREON FOR BIOCONVERSION OF RENEWABLE VEGETAL RAW MATERIAL INTO SUGARS | 2019 |
|
RU2741078C1 |
| PENICILLIUM VERRUCULOSUM FUNGUS STRAIN AS PRODUCER OF COMPLEX OF PHYTASE A AND ENDO-1,4-β-XYLANASE E AND ENZYME PREPARATION BASED ON IT FOR USE AS ADDITIVE IN FEED | 2023 |
|
RU2819918C1 |
| FUNGAL STRAIN Penicillium verruculosum B10 EGII PRODUCER OF ENDO-1,3/1,4-β-GLUCANASE, CELLULASE, β-GLUCOSIDASE AND XYLANASE AND METHOD OF PRODUCTION OF FODDER COMPLEX ENZYME PREPARATION | 2012 |
|
RU2532840C2 |
