FIELD: chemistry.
SUBSTANCE: invention relates to biotechnology, in particular to the microbiological industry, and represents a method of obtaining complex multienzymatic medications with cellobiohydrolase, endoglucanase, β-glucosidase (cellobiase) activities by the cultivation of recombinant strains of mycelium fungi of the Penicillium verruculosum family, transformed by linear fusion-construction, which represents successively connected through a linker homological genes and a heterologous gene of carbohydrases, such as cellobiohydrolase I, endogluconase and beta-glucosidase.
EFFECT: invention makes it possible to obtain effective the multienzymatic medication of a specified composition.
3 cl, 2 tbl, 3 dwg
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Authors
Dates
2015-05-10—Published
2013-09-10—Filed