GENETIC FUSION-CONSTRUCTION FOR PROVIDING EXPRESSION OF MULTIENZYMATIC COMPLEX OF CARBOHYDRASES IN CELLS OF MYCELIUM FUNGUS Penicillium verruculosum, APPLIED AS HOST, METHOD OF OBTAINING RECOMBINANT STRAIN OF FUNGUS Penicillium verruculosum AND METHOD OF OBTAINING ENZYMATIC MEDICATION ON ITS BASE Russian patent published in 2015 - IPC C12N9/00 

Abstract RU 2550044 C2

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology, in particular to the microbiological industry, and represents a method of obtaining complex multienzymatic medications with cellobiohydrolase, endoglucanase, β-glucosidase (cellobiase) activities by the cultivation of recombinant strains of mycelium fungi of the Penicillium verruculosum family, transformed by linear fusion-construction, which represents successively connected through a linker homological genes and a heterologous gene of carbohydrases, such as cellobiohydrolase I, endogluconase and beta-glucosidase.

EFFECT: invention makes it possible to obtain effective the multienzymatic medication of a specified composition.

3 cl, 2 tbl, 3 dwg

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RU 2 550 044 C2

Authors

Sinitsyn Arkadij Pantelejmonovich

Rozhkova Aleksandra Mikhajlovna

Zorov Ivan Nikitich

Sinitsyna Ol'Ga Arkad'Evna

Proskurina Ol'Ga Vladimirovna

Korotkova Ol'Ga Genrikhovna

Bushina Ekaterina Vjacheslavovna

Okunev Oleg Nikolaevich

Matys Veronika Jur'Evna

Koshelev Anatolij Vladimirovich

Dates

2015-05-10Published

2013-09-10Filed