METHOD OF BIOLOGICAL TISSUES ANALYSIS FOR PROTOPORPHYRIN FRACTION Russian patent published in 2009 - IPC A61B10/00 G01N33/52 

FIELD: medicine; surgery.

SUBSTANCE: method involves spectroscopy with helium-neon laser-induced (632.8 nm) fluorescence excitation and recording of autofluorescence spectra as a data array consisting of fluorescence strengths for sequence of wave lengths 649 nm to 850 nm in pitch 0.43 nm {Z₁, Z₂, Z₃…Z_n}. It is combined with forming a data array consisting of fluorescence strengths of keratin {Y₁, Y₂, Y₃…Y_n} and portoporphyrin IX {X₁, X₂, X₃…X_n} for the same wavelengths. Portoporphyrin IX fraction is defined as proportional to specific fraction of its fluorescence within total autofluorescence spectrum calculated by formula: where {k₁, k₂…k_n} and {l₁, l₂…l_n} are calculated by formulas: where α and β are calculated by formulas: where {a₁, a₂…a_n}, {b₁ b₂…b_n}, {ab₁ ab₂…ab_n}, {f₁ f₂…f_n}, {fa₁ fa₂…fa_n}, {fb₁ fb₂…fb_n} are calculated by formulas:

where Xav, Yav and Zav are calculated by formulas: X_av = (X₁+X₂+X₃…X_n)/n; Y_av = (Y₁+Y₂+Y₃…Y_n)/n; Z_av = (Z₁+Z₂+Z₃…Z_n)/n, where n is a number of records in the data arrays consisting fluorescence intensities.

EFFECT: method allows for biological tissues analysis for portoporphyrin IX fraction with elimination of errors related to complex compound character of total autofluorescence spectrum.

2 dwg, 2 ex