FIELD: chemistry.
SUBSTANCE: invention relates to biotechnology and can be used for producing esters of such compounds as carbohydrates, proteins, protein subunits and hydroxyacids. The method involves mixing an acyl group donor, acyl group acceptor and water with formation of a medium with high water content, containing 5 to 98% water. The acyl group donor used is a lipid substrate, chosen from phospolipid, lysophospholipid, triacylglyceride, diglyceride, glycolipid or lysoglycolipid, and the acyl group acceptor used is carbohydrate, protein, protein subunitor hydroxyacid. The mixture is brought into contact with lipidacyltransferase, which catalyses alcoholysis and/or re-esterification and is an enzyme which has acyltransferase activity, containing a GDSX fragment of amino acid sequence, where X stands for one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S.
EFFECT: invention allows for obtaining one or more carbohydrate esters, protein esters, esters of protein subunits or hydroxyacid esters using lipidacyltransferase.
18 cl, 51 dwg, 10 tbl, 13 ex
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RU2548805C2 |
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PROTEIN | 2005 |
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RU2380414C2 |
COMPOSITION AND METHOD OF PRODUCING FRAGRANT ESTER | 2008 |
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RU2511409C2 |
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RU2479628C2 |
Authors
Dates
2009-10-27—Published
2004-01-15—Filed