METHOD OF PRODUCING CARBOHYDRATE ESTER, PROTEIN ESTER, ESTER OF PROTEIN SUBUNITS OR HYDROXYACID ESTER USING LIPIDACYLTRANSFERASE Russian patent published in 2009 - IPC C12N1/00 C12N9/10 C12P7/62 C12N9/18 C12P7/64 

Abstract RU 2371474 C2

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology and can be used for producing esters of such compounds as carbohydrates, proteins, protein subunits and hydroxyacids. The method involves mixing an acyl group donor, acyl group acceptor and water with formation of a medium with high water content, containing 5 to 98% water. The acyl group donor used is a lipid substrate, chosen from phospolipid, lysophospholipid, triacylglyceride, diglyceride, glycolipid or lysoglycolipid, and the acyl group acceptor used is carbohydrate, protein, protein subunitor hydroxyacid. The mixture is brought into contact with lipidacyltransferase, which catalyses alcoholysis and/or re-esterification and is an enzyme which has acyltransferase activity, containing a GDSX fragment of amino acid sequence, where X stands for one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S.

EFFECT: invention allows for obtaining one or more carbohydrate esters, protein esters, esters of protein subunits or hydroxyacid esters using lipidacyltransferase.

18 cl, 51 dwg, 10 tbl, 13 ex

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RU 2 371 474 C2

Authors

Krehij Arno De

Madrid Susan Mampusta

Mikkel'Sen Jern Dal'Gor

Seeh Jern Borkh

Dates

2009-10-27Published

2004-01-15Filed