METHOD OF DIAGNOSING ABNORMAL CHROMATIN PACKING IN MALE STERILITY AND DIAGNOSTIC AGENT FOR DETERMINING MALE STERILITY Russian patent published in 2016 - IPC G01N33/52

Abstract RU 2582914 C2

FIELD: medicine.

SUBSTANCE: invention relates to medicine and veterinary science, specifically to auxiliary reproductive technologies, particularly, to increase of frequency of pregnancy, and can be used in treating infertility within extracorporeal fertilisation and homologous insemination programs. Invention represents a diagnostic technique for abnormal chromatin packing in male sterility. Method according to invention for determining amount of sperm cells, comprises feeding aliquot of ejaculate into test tube with a cover, spermatozoa are deposited by centrifugation, precipitate is washed once with sodium phosphate buffer, separating precipitate by centrifuging, acidifying with diluted perchloric acid, boiling in a water bath and material is deposited by centrifugation, followed by determination of DNA concentration in precipitate.

EFFECT: technical result achieved by implementing disclosed invention consists in improvement of accuracy of determining abnormal chromatin packing in male sterility.

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RU 2 582 914 C2

Authors

Kirjanov Gleb Ivanovich

Poljakov Vladimir Jurevich

Kintsurashvili Lija Nodarevna

Dates

2016-04-27—Published

2014-03-06—Filed