EXPRESSION PLASMID VECTOR OF MONOCISTRONIC EXPRESSION OF RECOMBINANT PROTEINS IN MAMMALIAN CELLS, CELL LINE OF MAMMALS-PRODUCERS OF RECOMBINANT PROTEIN, METHOD OF OBTAINING RECOMBINANT PROTEIN Russian patent published in 2015 - IPC C12N15/06 C12N15/09 

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology and genetic engineering. Claimed is expression plasmid vector for monocistronic heterologous expression of recombinant proteins in cells of Chinese hamster ovary (CHO), possessing property of high-frequency integration of expression cassette in said cells, in the following sequence containing region of initiation of plasmid pUC replication with functional gene of ampicilline resistance; site of Epstein-Barr virus terminal repetition (EBVTR), functional promoter of elongation factor 1 alpha gene of Chinese hamster, flanked with 5' untranslated region of said gene; site for cloning open reading frame of recombinant protein (polylinker); functional terminator of elongation factor 1 alpha gene of Chinese hamster, flanked with 3' untranslated region of said gene, promoter, functioning in mammalian cells; gene of antibiotic resistance; and functional terminator of antibiotic resistance gene. Also claimed is line of cells - recombinant protein producers and method of obtaining recombinant protein with application of said cells.

EFFECT: invention makes it possible to increase stability and productivity of expression systems of recombinant proteins.

13 cl, 7 dwg, 6 tbl, 8 ex