FIELD: biotechnology.
SUBSTANCE: group of inventions relates to biotechnology. Proposed is a recombinant strain of fungus Penicillium verruculosum PVERINU13 BKPMF-1292 and the strain of Penicillium verruculosum fungus PVERINU18 BKPMF-1291. These strains are producers of a complex of cellulolytic enzymes, including cellobiohydrolases I and II, endoglucanase, beta-glucosidase of fungus Penicillium verruculosum and heterologous exoinulinase from Aspergillus awamori. Proposed are variants of a method for producing an enzyme preparation of cellulases, such as cellobiohydrolases I and II, endoglucanase, beta-glucosidase of fungus Penicillium verruculosum, and exoinulinase and consisting in cultivation of the strain of fungus Penicillium verruculosum PVERINU13 BKPMF-1292 or the strain of fungus Penicillium verruculosum PVER18 BKPMF-1291 , followed by concentration of the culture liquid and drying.
EFFECT: group of inventions provides the exoinulinase and cellulose preparation with high activity and can be used in biotechnology when converting any type of inulin containing raw materials into glucose and fructose.
4 cl, 4 dwg, 2 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| NEW RECOMBINANT STRAIN (VERSIONS) OF MYCELIAL FUNGUS PENICILLIUM VERRUCULOSUM, AND ENZYMATIC AGENT (VERSIONS) INTENDED FOR HYDROLYSIS OF FRUIT RAW MATERIAL, AND METHOD OF ITS PRODUCTION | 2014 |
|
RU2574206C1 |
| PENICILLIUM CANESCENS MTCBHI FUNGUS STRAIN PRODUCER OF THERMALLY STABLE CELLOBIOHYDROLASE I AND AN ENZYME PREPARATION BASED THEREON FOR BIOCONVERSION OF RENEWABLE VEGETAL RAW MATERIAL INTO SUGARS | 2019 |
|
RU2741078C1 |
| GENETIC FUSION-CONSTRUCTION FOR PROVIDING EXPRESSION OF MULTIENZYMATIC COMPLEX OF CARBOHYDRASES IN CELLS OF MYCELIUM FUNGUS Penicillium verruculosum, APPLIED AS HOST, METHOD OF OBTAINING RECOMBINANT STRAIN OF FUNGUS Penicillium verruculosum AND METHOD OF OBTAINING ENZYMATIC MEDICATION ON ITS BASE | 2013 |
|
RU2550044C2 |
| METHOD FOR PRODUCING AN ENDO-INULINASE AND SUCRASE ENZYME PREPARATION BY CULTIVATION OF RECOMBINANT MYCELIAL FUNGUS PENICILLIUM CANESCENS Sopp INUA3 STRAIN | 2015 |
|
RU2605635C1 |
| GENETIC CONSTRUCT FOR EXPRESSION OF A COMPLEX OF ENZYMES OF ENDOGLUCANASES AND XYLANASES IN THE CELLS OF THE FUNGUS PENICILLIUM VERRUCULOSUM AND METHOD FOR OBTAINING COMPLEX ENZYME PREPARATIONS BASED ON IT, INTENDED FOR FODDER PRODUCTION | 2017 |
|
RU2653429C1 |
| PENICILLIUM VERRUCULOSUM FUNGUS STRAIN AS PRODUCER OF COMPLEX OF PHYTASE A AND ENDO-1,4-β-XYLANASE E AND ENZYME PREPARATION BASED ON IT FOR USE AS ADDITIVE IN FEED | 2023 |
|
RU2819918C1 |
| STRAIN OF PENICILLIUM VERRUCULOSUM PRODUCER OF PHYTASE AND ENDO-1,4-β-GLUCANASE II COMPLEX AND ENZYME PREPARATION BASED ON IT FOR USE AS ADDITIVE IN FEED | 2022 |
|
RU2810538C2 |
| PENICILLIUM VERRUCULOSUM MX-73 FUNGUS STRAIN PRODUCER OF MODIFIED XYLANASE E WITH HIGH HEAT STABILITY, ENZYME PREPARATION BASED THEREON FOR USE IN FOOD AND FEED INDUSTRY AND METHOD FOR PRODUCTION THEREOF | 2018 |
|
RU2711578C1 |
| STRAIN OF FUNGUS PENICILLIUM VERRUCULOSUM EE-105, PRODUCER OF A COMPLEX OF HIGHLY EFFECTIVE ENDOGLUCANASES AND AN ENZYME PREPARATION BASED THEREON FOR USE AS A FODDER ADDITIVE IN CEREAL FODDERS | 2018 |
|
RU2696036C1 |
| FUNGAL STRAIN Penicillium verruculosum B10 EGII PRODUCER OF ENDO-1,3/1,4-β-GLUCANASE, CELLULASE, β-GLUCOSIDASE AND XYLANASE AND METHOD OF PRODUCTION OF FODDER COMPLEX ENZYME PREPARATION | 2012 |
|
RU2532840C2 |
