CROSS-LINKED LIPASE CRYSTALS, COMPOSITION BASED THEREON AND METHOD FOR MAKING THEREOF Russian patent published in 2010 - IPC C12N9/20 C12P21/00 

FIELD: medicine.

SUBSTANCE: invention covers lipase crystals cross-linked with a coupling agent able for insolubility-to-solubility transition and release of soluble lipase resulted from changing the pH value of a medium that surrounds said crystal. According to one version of the invention, the cross-linked lipase crystals are able to release the lipase activity at the controlled rate 0.1% to 100% of a crystalline material a day. The cross-linked lipase crystals are grown by the method that involves interaction of lipase crystals with the coupling agent in conditions sufficient to initiate cross-link of crystals. There is also offered lipase delivery system that includes a cross-linked lipase crystal and consists of microparticles, where the cross-linked lipase crystals have the greatest dimension approximately 0.01 to approximately 500 mcm. Herewith the cross-linked lipase crystal forms provide approximately 50% to approximately 90% of lipase weight content. There is offered composition with controlled rate of lipase activity containing the cross-linked lipase crystals and a acceptable carrier or adjuvant.

EFFECT: cross-linked lipase crystals in the composition are practically insoluble in storage and are able to release lipase activity in vivo at controlled rate.

30 cl, 9 dwg, 36 tbl, 55 ex