VERSIONS OF BACILLUS LICHENIFORMIS ALPHA-AMYLASE WITH INCREASED THERMOSTABILITY AND/OR DECREASED CALCIUM DEPENDENCE Russian patent published in 2012 - IPC C12N9/26 C12N9/28 C12N15/56 C12N15/75 C08B30/12 

FIELD: chemistry.

SUBSTANCE: described is a version of Bacillus licheniformis alpha-amylase having an amino acid sequence which is at least 90% identical the SEQ ID NO: 4 sequence and contains a substitution of S239Q, corresponding to SEQ ID NO:4 given in the description. Provided are a nucleic acid which codes said version; an expression vector containing said nucleic acid; and a host cell containing said vector or nucleic acid. Described are compositions containing the disclosed alpha-amylase and used for starch liquefaction, starch saccharification, starch processing, textile desizing, baking, as well as detergent compositions and an additive. Disclosed is a method of producing ethanol comprising the following steps: a) using the starch liquefaction composition on ground maize or starch suspension; b) starch saccharification; c) fermentation of the saccharified starch; d) obtaining ethanol.

EFFECT: invention enables to obtain compositions containing alpha-amylase, having high thermostability and melting point.

43 cl, 3 tbl, 9 dwg, 6 ex