FIELD: chemistry.
SUBSTANCE: mixture of chondroitin-6-sulphate and dermatan sulphate extracted from umbilical cords is oxidised with 2,2,6,6-tetramethylpiperidine-1-oxyl-NaClO-NaBr at pH 10.2 and temperature 0-5°C for 1 hour with subsequent precipitation of oxidation products with three ethanol objects and their separation on a chromatographic column with DEAE-cellulose using eluent in form of aqueous solutions of NaCl with increasing concentration 0.2→0.6 M in increment of 0.05.
EFFECT: obtaining pure chondroitin-6-sulphate; the rest of the products are hybrid polysaccharides consisting of chondroitin-6-sulphate and dermatan sulphate oxidised on the primary groups.
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Authors
Dates
2010-03-10—Published
2008-02-20—Filed