FIELD: medicine.
SUBSTANCE: method facilitates linkage of sequences, coding immunoglobulin variable regions, T-cells receptors or B-cells receptors. Method is instrument of higher effectivity for making sequence data libraries. Capability of multiple RT-PCR with chain extension by interruption with employment of matrix, derived from single cell, provides highly effective creation of sister pairs libraries.
EFFECT: method is effective for linkage of two or few nucleotide sequences, coding domens or subunits of heteromeric protein as a result of single reaction performance.
51 cl, 25 dwg, 27 tbl, 14 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF CLONING COGNATE ANTIBODIES | 2008 |
|
RU2459868C2 |
| IDENTIFICATION OF POLYNUCLEOTIDES ASSOCIATED WITH SAMPLE | 2012 |
|
RU2644681C2 |
| COMPOSITIONS OF RECOMBINANT ANTI-EPIDERMAL GROWTH FACTOR RECEPTOR ANTIBODIES | 2008 |
|
RU2488596C2 |
| QUICK METHOD OF CLONING AND EXPRESSION OF RELATED ANTIBODY VARIABLE REGION GENE SEGMENTS | 2012 |
|
RU2612910C2 |
| IDENTIFICATION OF AFFINELY-MATURE HUMAN ANTIBODIES | 2012 |
|
RU2636045C2 |
| DNA MUTAGENESIS BY DISORDERED FRAGMENTATION AND SECONDARY ASSEMBLY | 1995 |
|
RU2157851C2 |
| ANTIBODY OR FRAGMENT THEREOF WITH HIV-NEUTRALISING ACTIVITY | 2005 |
|
RU2380378C2 |
| ANTIBODY-PRODUCING NON-HUMAN MAMMALS | 2009 |
|
RU2559524C2 |
| RABIES VIRUS-SPECIFIC HUMAN NEUTRALIZING MONOCLONAL ANTIBODY AND NUCLEIC ACID AND METHODS ASSOCIATED WITH THEREOF | 2001 |
|
RU2272809C2 |
| PRODUCING NON-HUMAN MAMMALIAN ANTIBODIES | 2009 |
|
RU2731084C2 |
