FIELD: medicine.
SUBSTANCE: invention relates to field of biotechnology. Claimed is transgenic animal, representing mouse or rat, into whose genome inserted is nucleic acid, coding rebuilt variable region of light human immunoglobulin chain, functionally connected with constant region of said animal immunoglobulin and functionally connected with promoter, enabling expression of transgenic light chain in mammalian B-cells in such a way that it forms pairs with different endogenic heavy chains, enabling production of antibodies. Methods of producing antibodies with application of transgenic animal, B-cell of transgenic animal, application of animal and its B-cell for production of antibodies and method of obtaining transgenic animal in accordance with invention are also claimed.
EFFECT: invention makes it possible to produce antibodies, containing light chain with variable area of human immunoglobulin with different heavy chains of animal immunoglobulin.
24 cl, 27 dwg, 11 tbl, 22 ex
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