FIELD: chemistry.
SUBSTANCE: method relates to a procedure for linking cognate pairs of VH and VL encoding sequences from a population of cells enriched in particular surface antigen markers. The linking procedure involves a multiplex molecular amplification procedure capable of linking nucleotide sequences of interest in a particular polymerase chain reaction (multiplex PCR).
EFFECT: method is particularly effective for generation of cognate pair libraries as well as combinatorial libraries of variable region encoding sequences from immunoglobulins.
25 cl, 7 dwg, 6 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| CONCERNED SEQUENCES LINKAGE METHOD | 2004 |
|
RU2392324C2 |
| IDENTIFICATION OF POLYNUCLEOTIDES ASSOCIATED WITH SAMPLE | 2012 |
|
RU2644681C2 |
| COMPOSITIONS OF RECOMBINANT ANTI-EPIDERMAL GROWTH FACTOR RECEPTOR ANTIBODIES | 2008 |
|
RU2488596C2 |
| METHOD FOR AMPLIFICATION OF NUCLEIC ACIDS OF HEAVY AND LIGHT CHAINS OF HUMAN IMMUNOGLOBULINS FOR THE CREATION OF RECOMBINANT ANTIBODIES, PRIMER COMPOSITIONS (VARIANTS) | 2020 |
|
RU2775914C2 |
| HIGH THROUGHPUT SEQUENCING OF POLYNUCLEOTIDE LIBRARIES AND TRANSCRIPTOME ANALYSIS | 2018 |
|
RU2790291C2 |
| METHOD AND SET FOR DETECTING GENE ENCODING MEMBRANE-BOUND PROTEIN, VECTOR (VARIANTS) | 1999 |
|
RU2246538C2 |
| QUICK METHOD OF CLONING AND EXPRESSION OF RELATED ANTIBODY VARIABLE REGION GENE SEGMENTS | 2012 |
|
RU2612910C2 |
| DISPLAY SYSTEM BASED ON FULL LENGTH ANTIBODY FOR EUKARYOTIC CELLS, AND ITS APPLICATION | 2012 |
|
RU2625033C2 |
| MOUSE PRODUCING BINDING PROTEINS CONTAINING VL-DOMAINS | 2011 |
|
RU2612903C2 |
| ANTIBODIES RECOGNISING EPITOPE ON CD43 AND CEA EXPRESSED ON MALIGNANT CELLS AND METHODS FOR USING THEM | 2008 |
|
RU2528738C2 |
