FIELD: chemistry.
SUBSTANCE: method of producing isobutanol involves obtaining a recombinant microbial host cell which contains a fermentative isobutanol channel which contains DNA molecules which code a set of polypeptides which catalyse the following substrate conversions to a product: i) pyruvate to acetolactate; ii) acetolactate to 2,3-dihydroxyisovalerate; iii) 2,3-dihydroxyisovalerate to α-ketoisovalerate; iv) α-ketoisovalerate to isobutyraldehyde; and v) isobutyraldehyde to isobutanol, and bringing the host cell into contact with the fermented carbon substrate in a fermentation medium under conditions at which isobutanol is produced.
EFFECT: isobutanol with high efficiency.
54 cl, 1 dwg, 13 tbl, 22 ex
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