MUTANT GENE Δ ENCODING POLYPEPTIDE ELICITING ACTIVITY OF HUMAN GAMMA-INTERFERON, RECOMBINANT PLASMID Δ PROVIDING EXPRESSION OF Δ GENE IN ESCHERICHIA COLI AND STRAIN ESCHERICHIA COLI - A PRODUCER OF POLYPEPTIDE ELICITING ACTIVITY OF HUMAN GAMMA-INTERFERON Russian patent published in 1998 - IPC

Abstract RU 2105813 C1

FIELD: biotechnology, genetic engineering. SUBSTANCE: gene of mutant human gamma-interferon IFNΔ-10 is obtained by oligonucleotide-directed mutagenesis and this gene is used for replacement gene of natural gamma-interferon in plasmid pIF-γ-trp2 by recombination and obtained plasmid pIFΔ-10 is used for competent cells E. coli MH-1 transformation. Obtained strain-producer E. coli VKPM B-6663 provides inducible synthesis of polypeptide eliciting activity of human gamma-interferon at expression level up to 40% of total cellular protein. Mutant gamma-interferon is resistant to blood proteases action. EFFECT: improved quality of mutant gamma-interferon. 3 cl, 4 dwg

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RU 2 105 813 C1

Authors

Tat'Kov S.I.

Il'Ichev A.A.

Smirnova O.Ju.

Zakabunin A.I.

Dates

1998-02-27—Published

1995-10-12—Filed