FIELD: medicine.
SUBSTANCE: method provides introduction of adrenaline 0.5-5.0 mM in distilled water to prepare adrenaline solution. Fresh Gracilaria verrucosa algae are added to the prepared adrenaline solution and incubated for 1-2 hour in light illumination of intensity 100-300 mcE/m2s and temperature no more than 4°C. After incubation, the prepared solution is separated from algae, acidified to pH 2.5-3.0 and passed through a grafted hydrocarbon nonpolar modified silica gel column. Then it is eluted with water organic solvents and divided on individual prostaglandides by high-yield liquid chromatography.
EFFECT: method allows for higher yield of F2α prostaglandin.
2 cl, 9 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF OBTAINING PROSTAGLANDINS FROM ALGA GRACILARIA VERRUCOZA | 2008 |
|
RU2383354C1 |
| METHOD OF SYNTHESIS OF PROSTAGLANDINS TYPE e, OR THEIRS DERIVATIVES TYPE A, B, F, OR METHYL ESTERS OF PROSTAGLANDIN TYPE 1 MULTIPLE LABELED WITH TRITIUM | 1988 |
|
SU1646252A3 |
| METHOD OF SYNTHESIS OF F-PROSTAGLANDIN LABELED WITH TRITIUM | 1990 |
|
SU1774660A1 |
| METHOD FOR OBTAINING OF ARAHIDONIC ACID FROM MARINE RED GRACILARIA ALGAE | 2016 |
|
RU2620164C1 |
| METHOD OF PROSTAGLANDIN F3α PREPARING | 1990 |
|
RU1774659C |
| METHOD OF OBTAINING LUTEOLYN 7,3'-DISULPHATE | 2010 |
|
RU2432960C1 |
| METHOD FOR PRODUCING GEL-FORMING POLYSACCHARIDE AGAR FROM RED ALGAE | 2019 |
|
RU2770383C2 |
| METHOD OF HIGHLY PURIFIED MONOCOMPONENT INSULIN PREPARING | 2000 |
|
RU2186581C2 |
| METHOD FOR OBTAINING THROMBOXAN B LABELLED WITH TRITIUM | 1990 |
|
SU1732514A1 |
| METHOD OF PREPARING OF THROMBOXANE B LABELED WITH TRITIUM | 1990 |
|
SU1732513A3 |
