METHOD FOR POLYSACCHARIDE ANALYSIS IN HERB OF VIOLET SPECIES Russian patent published in 2013 - IPC G01N33/15 

FIELD: medicine.

SUBSTANCE: method for polysaccharide analysis in herbs of Violet species involving grinding of raw materials, water extraction of a polysaccharide complex when heated, addition of an agent, filtration and polysaccharide quantification by formula, differs by the fact that the raw material is Viola uniflora herb; water extraction lasts for 1 hour in ratio of the raw material : the extractant as 1:50; the extract is filtered, and the filtrate is added with 8% hydrochloric acid in ratio 1:1; the mixture is kept for 2 hours on a boiling water bath, then cooled and reduced to pH=6.5-7.0; thereafter the mixture is added with 1% picric acid and 20% sodium carbonate in ratio 1:3; the prepared mixture is kept on a water bath for 30 minutes, then filtered; it is followed by spectrophotometric analysis of the filtrate in a tray of the absorption layer thickness of 1 am at wave length λ=460±2 nm, while polysaccharides are quantified at a glucose basis by formula.

EFFECT: method enables assessing the quality of medical plants by quantifying accurately polysaccharides contained in herbs of Violet species.

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