METHOD FOR MYCOBACTERIUM TUBERCULOSIS BEIJING GENOTYPE DETECTION Russian patent published in 2010 - IPC C12Q1/68 

FIELD: medicine.

SUBSTANCE: conventional method for Mycobacterium tuberculosis Beijing genotype strain is spoligotyping based on the structural analysis of M tuberculosis chromosome in the direct repeats divided by variable spacers. A method for length polymorphism heteroduplex analysis of DNA restriction fragments containing an insertion element IS6110 (method IS6110-RFLP), applied to mycobacterium tuberculosis strain differentiation, including Beijing genotype strain, cannot be used for Beijing genotype characterisation as a single gene family because of variety of the produced restriction profiles. The invention detects PCR amplified DNA specific fragments (genome regions between proximal copies of the element IS6110) of size 290-pn and 470-pn for "ancient" (atypical) Beijing strains or of size 260-pn, 290-pn and 470-pn for "modern" (typical) Beijing M. tuberculosis strains. The results are analysed by PCR product electrophoresis in the horizontal agarose mini-gel.

EFFECT: fast and reliable mycobacterium tuberculosis Beijing genotype detection.

6 dwg, 2 ex