FIELD: medicine.
SUBSTANCE: invention refers to medicine, particularly phthisiology, and aims at detecting isolates of Mycobacterium tuberculosis Beijing 94-32-cluster. Availability of nucleotide substitution G>A in sigE gene in position 294 by means of PCR in real time format using oligonucleotide primers and fluorescent-labeled probes. PCR results are evaluated by recording a fluorescence signal on a HEX channel at wavelength of 555 nm, and with exponential accumulation it is determined whether M. tuberculosis belongs to Beijing 94-32-cluster. When detecting the fluorescence signal on the control FAM-channel with wave length 510 nm, the strain belongs to another genotype of M. tuberculosis.
EFFECT: invention provides fast and reliable detection of tuberculosis mycobacteria Beijing 94-32-cluster genotype.
1 cl, 3 dwg, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DETECTION OF MYCOBACTERIUM TUBERCULOSIS BEIJING 1071-32-CLUSTER GENOTYPE IN REAL-TIME FORMAT | 2021 |
|
RU2768021C1 |
| METHOD OF DETECTING PHYLOGENETIC SUBLINES OF BEIJING MYCOBACTERIUM TUBERCULOSIS GENOTYPE IN REAL TIME FORMAT | 2020 |
|
RU2743365C1 |
| METHOD OF DETECTING MYCOBACTERIUM TUBERCULOSIS BEIJING 14717-15-CLUSTER GENOTYPE | 2022 |
|
RU2812351C1 |
| METHOD FOR DETECTING MYCOBACTERIA TUBERCULOSIS CENTRAL ASIAN EPIDEMIC CLUSTER BEIJING GENOTYPE | 2019 |
|
RU2735415C1 |
| METHOD OF IDENTIFICATION OF MYCOBACTERIA OF TUBERCULOSIS OF THE BEIJING GENOTYPE B0 CLUSTER IN REAL TIME FORMAT | 2017 |
|
RU2684314C2 |
| METHOD FOR DETERMINING THE SUBLINES OF THE TUBERCULOSIS CAUSATIVE AGENT OF THE L2 beijing LINE ON BIOLOGICAL MICROCHIPS | 2022 |
|
RU2790296C1 |
| METHOD FOR REAL-TIME DETECTION OF BEIJING GENOTYPE MYCOBACTERIUM TUBERCULOSIS | 2011 |
|
RU2528866C2 |
| METHOD FOR DETECTING TUBERCULOUSIS MYCOBACTERIA OF GENETIC CLUSTER Beijing B0/W148 | 2013 |
|
RU2551764C2 |
| (4-(3-HYDROXYPHENYL)PIPERAZINE-1-YL)(5-NITROFURAN-2-YL)METHANON, WHICH HAS ANTITUBERCULAR ACTIVITY AGAINST THE CAUSATIVE AGENT OF MULTIDRUG-RESISTANT TUBERCULOSIS, AND THE METHOD FOR ITS PREPARATION | 2021 |
|
RU2784399C1 |
| METHOD FOR MYCOBACTERIUM TUBERCULOSIS BEIJING GENOTYPE DETECTION | 2008 |
|
RU2405836C2 |
