METHOD OF DETECTING MYCOBACTERIUM TUBERCULOSIS BEIJING 14717-15-CLUSTER GENOTYPE Russian patent published in 2024 - IPC C12Q1/68 C12Q1/6844 C12Q1/686 C12Q1/6876 

FIELD: molecular biology.

SUBSTANCE: method of detecting the genotype of Mycobacterium tuberculosis Beijing 14717-15 cluster is described, the said method is characterized by the following: the presence of a nucleotide substitution in the Rv1293 gene at position 303G>T is detected using PCR using oligonucleotide primers, followed by treatment of the PCR product with the restriction endonuclease HhaI and electrophoresis of the restriction products in agarose gel. In the case of the Rv1293 303G>T mutation, specific for the genetic cluster Beijing 14717-15 cluster, the PCR product remains unchanged (205 bp), and in the presence of two restriction fragments of length 120 and 85 bp, the strain belongs to any other genotype of M. tuberculosis.

EFFECT: speed (less than one day from the time of DNA isolation), simple and unambiguous interpretation of the results, the ability to analyse large collections of M. tuberculosis strains to assess their belonging to the Beijing 14717-15 cluster for diagnostic purposes and in population studies, as well as the ability to identify contamination and mixed infections.

1 cl, 4 dwg 3 ex