METHOD FOR DETECTION OF MYCOBACTERIUM TUBERCULOSIS BEIJING 1071-32-CLUSTER GENOTYPE IN REAL-TIME FORMAT Russian patent published in 2022 - IPC C12Q1/68 C12N15/00 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, medicine, namely phthisiology, and can be used for laboratory determination of mycobacterium tuberculosis belonging to the Beijing 1071-32-cluster genotype. A single nucleotide substitution C>T in the Rv0144 gene at position 222, specific for mycobacterium tuberculosis of the Beijing 1071-32 genotype, is detected using real-time PCR using specific fluorescently labeled probes to discriminate between wild and mutant Rv0144 222C/T alleles. The accumulation of the amplification product of a DNA fragment specific to Beijing 1071-32 cluster is recorded via the FAM fluorescence channel (510 nm), and a DNA fragment specific to a strain of any other M. tuberculosis genotype is recorded via the HEX channel (555 nm). If the Beijing 1071-32-cluster strain DNA is present in the sample, an exponential increase in the fluorescence signal via the FAM detection channel occurs between 20-35 PCR cycles and there is no fluorescence signal via the control HEX detection channel.

EFFECT: invention makes it possible to identify strains of this genotype, which is an important component of the diagnosis of tuberculosis and the choice of adequate treatment.

1 cl, 3 dwg, 3 ex