FIELD: medicine.
SUBSTANCE: invention represents recombinant plasmid DNA pBinPLUS-ARS-EPSPS sized 14.8 kbp providing gene expression of agrobacterial 5-enolpiruvoyl-shikimate-3-phosphat-synthetase under the control of a gene promoter of chlorella alpha-tubulin in transgene chlorella microalgae consisting of the following elements: DNA of vector plasmid pBinPLUS-ARS sized 12440 bp, Hindlll-BgIII of a DNA fragment sized 500 bp, containing a gene promoter of chlorella alpha-tubulin hlorelly, Bglll-Xbal of a DNA fragment sized 1610 bp, containing a coding sequence of EPSPS gene with a signal peptide, Xbal-Hindlll of a DNA fragment sized 230 bp, containing 35S terminator of cauliflower mosaic virus.
EFFECT: use of recombinant plasmid DNA pBinPLUS-ARS-EPSPS will allow to produce glyphosate-resistant transgene chlorella microalgae.
3 dwg, 2 tbl, 2 ex
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