FIELD: medicine, pharmaceutics.
SUBSTANCE: group of inventions refers to biotechnology, gene and protein engineering. There are engineered plasmids pCLm4/hygro-14D5 and pCHm2-14D5. The plasmids provide the synthesis in eukaryotic cells of polypeptides with light and heavy chain properties of a chimeric antibody, which are combined into the chimeric antibody ch14D5 class IgG1/kappa. The producing chimeric antibody ch14D5 recovered from the culture fluid of CHO-K1 cells transfected by plasmids pCLm4/hygro-14D5 and pCHm2-14D5 having a molecular weight of about 150 kDa. The antibody consists of two identical light chains, a constant portion of which corresponds to human kappa antibodies, and two identical heavy chains, a constant portion of which corresponds to human IgGI antibodies; having an amino acid sequence SEQ ID NO: 1 and SEQ ID NO: 2.
EFFECT: antibody is targeted to tick-borne encephalitis and provides the acute prevention of mice against tick-borne encephalitis.
3 cl, 7 dwg, 8 ex
| Title |
Year |
Author |
Number |
| PVEAL3-10H10CH PLASMID GENETIC CONSTRUCT, STRAIN OF CHO-K1-10H10CH RECOMBINANT CELL LINE AND 10H10CH CHIMERIC ANTIBODY AGAINST TICK-BORNE ENCEPHALITIS VIRUS PRODUCED BY THE INDICATED STRAIN OF CHO-K1-10H10CH CELL LINE |
2022 |
- Shanshin Daniil Vasilevich
- Nesmeyanova Valentina Sergeevna
- Shcherbakov Dmitrij Nikolaevich
- Loktev Valerij Borisovich
- Protopopova Elena Viktorovna
- Ilichev Aleksandr Alekseevich
|
RU2800471C1 |
| RECOMBINANT PLASMID DNA pCL1 ENCODING POLYPEPTIDE WITH PROPERTY OF HUMAN LIGHT CHAIN ANTIBODY AGAINST EBOLA VIRUS, RECOMBINANT PLASMID DNA pCH1 ENCODING POLYPEPTIDE WITH PROPERTY OF INDICATED ANTIBODY HEAVY CHAIN AND THEIR USING |
2004 |
- Shingarova Ljudmila Nikolaevna
- Tikunova Nina Viktorovna
- Jun Tat'Jana Ehverestovna
- Batanova Tat'Jana Aleksandrovna
- Aliev Tejmur Kantamirovich
- Boldyreva Elena Filippovna
- Nekrasova Oksana Vasil'Evna
- Polykhalova Ilona Vladimirovna
- Panina Anna Alekseevna
- Kirpichnikov Mikhail Petrovich
- Il'Ichev Aleksandr Alekseevich
- Sandakhchiev Lev Stepanovich
|
RU2285043C2 |
| RECOMBINANT PLASMID DNA pcL37 ENCODING POLYPEPTIDE WITH PROPERTIES OF HUMAN LIGHT CHAIN OF ANTIBODY AGAINST SMALLPOX VIRUS VACCINE, RECOMBINANT PLASMID DNA pcH37 ENCODING POLYPEPTIDE WITH PROPERTIES OF HEAVY CHAIN OF INDICATED ANTIBODY AND THEIR USING |
2006 |
- Tikunova Nina Viktorovna
- Shingarova Ljudmila Nikolaevna
- Jun Tat'Jana Ehverestovna
- Morozova Vera Vital'Evna
- Aliev Tejmur Kantamirovich
- Boldyreva Elena Filippovna
- Nekrasova Oksana Vasil'Evna
- Polykhalova Ilona Vladimirovna
- Panina Anna Alekseevna
- Kirpichnikov Mikhail Petrovich
- Il'Ichev Aleksandr Alekseevich
- Sandakhchiev Lev Stepanovich
|
RU2317330C2 |
| RECOMBINANT PLASMID DNA PTLS CONTAINING GENE OF SINGLE-STRANDED ANTIBODY AGAINST TICK-BORNE ENCEPHALITIS VIRUS AND STRAIN OF BACTERIUM ESCHERICHIA COLI AS PRODUCER OF SINGLE-STRANDED ANTIBODIES AGAINST TICK-BORNE ENCEPHALITIS VIRUS |
1998 |
- Nikolenko G.N.
- Tikunova N.V.
- Golovin S.Ja.
- Protopopova E.V.
- Loktev V.B.
- Il'Ichev A.A.
- Deev S.M.
|
RU2158309C2 |
| RECOMBINANT PLASMID DNA pSC13D6 CONTAINING GENE OF ONE-CHAINED ANTIBODY TO TICK-BORNE ENCEPHALITIS VIRUS, AND STRAIN OF BACTERIA Escherichia coli BL21 (DE3)/pSC13D6-PRODUCER OF ONE-CHAINED ANTIBODIES TO TICK-BORNE ENCEPHALITIS VIRUS, EXPRESSING VIRUS NEUTRALISING PROPERTIES |
2008 |
- Levanov Lev Nikolaevich
- Tikunova Nina Viktorovna
- Matveev Leonid Ehduardovich
- Goncharova Elena Pavlovna
- Jun Tat'Jana Ehverestovna
- Ryzhikov Aleksandr Borisovich
- Matveeva Vera Aleksandrovna
- Rikhter Vladimir Aleksandrovich
|
RU2378378C1 |
| RECOMBINANT PLAZMID DNA pCL2h-1I1G PROVIDING SYNTHESIS OF POLYPEPTIDE WITH PROPERTIES OF LIGHT CHAIN OF HUMAN ANTIBODY AGAINST INTERLEUKIN-18, RECOMBINANT PLAZMID DNA pCH2g-1I1G PROVIDING SYNTHESIS OF POLYPEPTIDE WITH PROPERTIES OF HEAVY CHAIN OF HUMAN ANTIBODY AGAINST INTERLEUKIN-18 AND RECOMBINANT HUMAN ANTIBODY fh1I1G WITH ABILITY TO BIND INTERLEUKIN-18 |
2014 |
- Tjumentseva Marina Alekseevna
- Tikunova Nina Viktorovna
- Babkina Irina Nikolaevna
- Matveev Andrej Leonidovich
- Bajkov Ivan Konstantinovich
- Kukharenko Ljudmila Aleksandrovna
|
RU2562857C1 |
| RECOMBINANT PLASMID DNA P7SHSHIS FOR PRODUCTION OF DIMERIC SINGLE-STRANDED ANTIBODY AGAINST TICK-BORNE ENCEPHALITIS VIRUS AND STRAIN OF ESCHERICHIA COLI AS PRODUCER OF DIMERIC FORM OF SINGLE-STRANDED ANTIBODY AGAINST TICK-BORNE ENCEPHALITIS VIRUS |
2000 |
- Nikolenko G.N.
- Tikunova N.V.
- Protopova E.V.
- Loktev V.B.
- Karpenko L.I.
- Konovalova S.N.
- Il'Ichev A.A.
|
RU2190017C2 |
| RECOMBINED PLASMID DNA CODING CHIMERIC ANTIBODY AGAINST HUMAN TUMOUR NECROSIS FACTOR-ALPHA, EUKARYOTIC CELL LINE PRODUCING CHIMERIC ANTIBODY, AND METHOD OF CHIMERIC ANTIBODY OBTAINMENT |
2013 |
- Aliev Tejmur Kantamirovich
- Balabashin Dmitrij Sergeevich
- Dolgikh Dmitrij Aleksandrovich
- Kirpichnikov Mikhail Petrovich
- Panina Anna Alekseevna
- Toporova Viktorija Aleksandrovna
|
RU2555533C9 |
| RECOMBINANT PLASMID DNA pFLAG-sc14D5a-Rm7 ENSURING SYNTHESIS OF HYBRID PROTEIN sc14D5a-Rm7, BACTERIA STRAIN Escherichia coli - PRODUCER OF HYBRID PROTEIN sc14D5a-Rm7, AND HYBRID PROTEIN sc14D5a-Rm7, COMBINING PROTEIN E OF TICK-BORNE ENCEPHALITIS MICE AND HAVING BIO-LUMINESCENCE ACTIVITY |
2014 |
- Frank Ljudmila Alekseevna
- Tikunova Nina Viktorovna
- Burakova Ljudmila Petrovna
- Bajkov Ivan Konstantinovich
- Kudrjavtsev Aleksandr Nikolaevich
- Morozova Vera Vital'Evna
|
RU2565545C1 |
| RECOMBINANT PLASMID DNA OF pBiPr-ABIgA2m1F16-ht TO OBTAIN RECOMBINANT IMMUNOGLOBULIN A OF ISOTYPE IgA2m1 |
2016 |
- Aliev Tejmur Kantamirovich
- Argentova Viktoriya Vitalevna
- Bokov Maksim Nikolaevich
- Votchitseva Yuliya Aleksandrovna
- Dementeva Irina Grigorevna
- Dolgikh Dmitrij Aleksandrovich
- Kirpichnikov Mikhail Petrovich
- Pozdnyakova Lyubov Petrovna
- Sveshnikov Petr Georgievich
- Toporova Viktoriya Aleksandrovna
|
RU2671477C2 |
