METHOD OF DIAGNOSING PATIENTS WITH BRAIN TUMOURS Russian patent published in 2011 - IPC A61B10/02 

FIELD: medicine.

SUBSTANCE: invention relates to field of medicine, namely to neurooncological surgery. In order to diagnose patients affected by brain tumours, histological analysis of tumour sample with determination of its type is carried out. Hystological sample of tumour is grinded in medium of liquids DMEM/F12, 10% fetal bull serum, 5% of L-glutalin and 5% of gentamycin, filtered and centrifuged. After that, phosphate-salt buffer is added to mixture, cell suspension is separated. Into board holes cell suspension and medium without cells are introduced. Content of board is incubated in thermostat at temperature 37°C in presence of 5% CO₂. After incubation, different cytostatics are introduced into holes with cell suspension and medium with their different concentration, after following incubation into each board hollow MTT solution in phosphate-salt buffer is added. Board content is incubated in thermostat and 100 mcM of isopropanol with 0.04 M HC 1 are added into each hole. Optic density in board holes at wave length 570 nm is measured. Results are evaluated comparing parametres of measured optic densities of medium and cell suspension with cytistatics and estimating % of cell death in tumour samples with different concentration of cytostastics.

EFFECT: method makes it possible to increase accuracy and efficiency of diagnostics by individual selection of cytostatics with determination of their concentration and with evaluation of sensitivity of cytostatic corticosteroid function.

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