NEUROTOXINS THAT DISPLAY REDUCED BIOLOGICAL ACTIVITY Russian patent published in 2016 - IPC C12N9/52 C12N1/21 C12N15/63 C07K14/33 C07K16/12 C12P21/02 A61K38/48 

FIELD: bioengineering.

SUBSTANCE: claimed polypeptide includes the signal of degradation in the light strand wherein the said reduced duration of a biological effect is the reduced duration of the biological effect as compared with the appropriate unmodified polypeptide neurotoxin and wherein the said degradation signal is selected from the group consisting of the following elements, i.e. internally or terminally introduced PEST motive, internally or terminally introduced motive of identification by an E3 ligase. A polynucleotide contains the series of a nucleic acid selected from the group consisting of the following elements, i.e. the series of nucleic acid SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15.

EFFECT: production of the polypeptide clostridial neurotoxin of the reduced duration of the biological effect on the subject.

16 cl, 2 tbl