METHOD OF PRODUCING PROTEOLYTICALLY PROCESSED POLYPEPTIDE Russian patent published in 2018 - IPC C07K14/33 C12N9/50 C12P21/06

Abstract RU 2673910 C2

FIELD: biotechnology.

SUBSTANCE: group of inventions relates to biotechnology, specifically to the use of Lys-C and a method for proteolytic processing of single-stranded botulinum neurotoxin serotype A (BoNT/A) to form an active, double-stranded botulinum neurotoxin serotype A (BoNT/A). Lys-C hydrolyses a single-stranded botulinum neurotoxin serotype A (BoNT/A) to form an active double-stranded botulinum neurotoxin serotype A (BoNT/A).

EFFECT: invention makes it possible to obtain active double-stranded BoNT/A without undesirable secondary cleavage products.

9 cl, 11 dwg, 8 ex, 2 tbl

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RU 2 673 910 C2

Authors

Rummel Andreas

Dates

2018-12-03—Published

2012-11-21—Filed