FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and represents a strain of Plantibacter flavus 3Kz, which is deposited in VKPM under registration number V-12248, which is a producer of methyl-dependent site-specific endonuclease PfsI, which recognises and splits both chains of DNA sequence , before central nucleotide N to form a single-nucleotide 5′-extending ends, in presence in given sequence of not less than seven C5-methyl-dependent cytosines.
EFFECT: invention enables to produce a bacterial strain, which produces methyl-dependent site-specific endonuclease PfsI with new site specificity.
1 cl, 2 dwg, 1 tbl, 3 ex
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Authors
Dates
2016-08-10—Published
2015-05-19—Filed