BIOLOGICAL DNA MARKER FOR DETERMINING POTATO TYPES, SET AND METHOD FOR GRADED IDENTIFICATION OF POTATOES Russian patent published in 2011 - IPC C12Q1/68 

FIELD: chemistry.

SUBSTANCE: to determine the type of a potato plant, DNA is extracted from fresh plant material and analysed through PCR analysis. PCR analysis is carried out using a set comprising a biological marker, a reaction mixture consisting of 160 mcM of each dinucleotide triphosphate dNTP, 1.6 mM MgCl₂, 0.3 mcM of each primer from the set, 0.3 units of a Taq-polymerase enzyme, 1x buffer for the Taq-polymerase and standard DNA of known potato types in amount of 50 ng. Polymorphous DNA markers are obtained and viewed using gel electrophoresis. In order to carry out titration of alleles extracted in the examined group, allele spectra of the analysed and control groups of samples are compared. The biological marker, which contains polymorphous DNA, is a specific nucleotide sequence obtained using a diagnostic set of three pairs of primers.

EFFECT: marker characterises the type of widespread and essential potato types, and samples of related species Solarium, most frequency used in crossing when creating new potato types.

5 cl, 3 dwg, 4 tbl, 3 ex