FIELD: medicine.
SUBSTANCE: method provides collection of an investigated material from a surface with a tampon made of an elastic finely porous material wetted in a solution inhibiting the development of other microorganisms. The collected investigated material with tampons is kept in the same solution for 18-24 hours. The investigated material is separated from a tampon and centrifuged. The precipitation is neutralised with 1% citric acid (in the ratio 1:1). No more than one-third of the investigated material is separate from the precipitation. The separated precipitation portion is placed on a phase-contrast slide to conduct phase-contrast microscopy. The residual precipitation is placed on a nutrient medium to be cultivated thereon if more exact quantitative assessment of mycobacterial pollution of the investigated surface is required.
EFFECT: invention allows reducing mycobacteria detection time.
2 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF DETECTION OF MYCOBACTERIUM FROM SURFACE | 1999 |
|
RU2180690C2 |
| METHOD OF DETECTION OF MYCOBACTERIA | 2001 |
|
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| METHOD FOR DETECTION OF TUBERCULOSIS MYCOBACTERIA IN URINE | 2002 |
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| METHOD FOR PRE-INOCULATION PROCESSING OF PATHOLOGICAL MATERIAL FOR MYCOBACTERIAL RECOVERY | 2013 |
|
RU2542460C1 |
| NUTRIENT MEDIUM FOR ISOLATING MYCOBACTERIA | 2005 |
|
RU2300571C2 |
| METHOD OF INDICATION OF TUBERCULOSIS MYCOBACTERIUM IN ATMOSPHERE AIR | 2000 |
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RU2162709C1 |
| METHOD FOR ISOLATING TUBERCULOSIS MYCOBACTERIA FROM AIR MEDIUM | 2001 |
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RU2221047C2 |
