METHOD FOR PRODUCING AGENT SHOWING ANTICANCER ACTIVITY, AND AGENT PRODUCED BY THIS METHOD (VERSIONS) Russian patent published in 2011 - IPC A61K36/07 A61K36/74 A61P35/00 

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to chemical-pharmaceutical industry. The developed versions of the method for producing the agent showing anticancer activity enables basidiomycete cultivation shorter from 2-3 weeks to 7 days and higher mycelium yield due to developing a composition of a nutrient medium, cultivation environment, sowing mycelium preparation, as well as higher efficacy of the agent. The method for producing the agent provides preparing the sowing basidiomycetes mycelium, preparing a processing medium, sowing the processing nutrient medium containing carbon and nitrogen sources, mineral salts with the prepared sowing mycelium, culturing basidiomycete to produce a submerged culture and recovering the agent showing anticancer activity. The sowing basidiomycetes mycelium is prepared in two stages, first of which is executed on a dense nutrient medium containing a corn extract, wheat water and agar, while the second one - on a fluid medium containing glucose, soya flour, potassium dihydrophosphate, magnesium sulphate and arachidonic acid. The prepared processing medium contains vegetable oil and soya flour in amounts 10-27 g/l and 16-27 g/l of water respectively, as well as potassium dihydrophosphate and magnesium sulphate in amounts 2.0-3.5 g/l and 0.2-0.4 g/l respectively. The basidiomycetes used are Ganoderma lucidum (Curt.:Fr.) P.Karst or Hypsizygus ulmarius (Bull.) Redhead, or Trametes versicolor (L.:Fr.) Pilat. The agent showing anticancer activity is recovered from the submerged culture prepared by cultivation or of the basidiomycete mycelium. The efficacy of the anticancer agent expressed by tumour growth inhibition in experiments in vivo makes 43-83%.

EFFECT: creation of the agent showing anticancer activity refers to the method for producing said agent by basidiomycetes cultivation in the submerged culture.

24 cl, 1 tbl, 22 ex