FIELD: food industry.
SUBSTANCE: method involves preparation of inoculation mycelium of basidiomycetes, preparation of productive nutrient medium and inoculation of the productive nutrient medium, containing carbohydrate and nitrogen source and mineral salts, with the prepared inoculation mycelium. Basidiomycetes cultivation with subsequent production of an immersion culture. The inoculation mycelium preparation is performed in a sterile nutrient medium containing glucose, soya flour, potassium dihydrogen phosphate, magnesium sulphate and arachidonic acid. The inoculation mycelium growing is performed at a temperature of 22-29°C during 2-6 days under aeration conditions with its subsequent homogenisation.
EFFECT: antineoplastic remedy produced from the basidiomycetes immersion culture is effective and the cultivation process duration is reduced from 2-3 weeks to one week; mycelium yield increases.
23 cl, 6 tbl, 12 ex
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Authors
Dates
2011-05-10—Published
2009-12-25—Filed