BASIDIOMYCETE INOCULATING MYCELIUM AND PREPARATION METHOD THEREOF Russian patent published in 2011 - IPC C12N1/14 A01G1/04 

FIELD: chemistry.

SUBSTANCE: method of preparing basidiomycete inoculating mycelium involves preparation of a sterile liquid culture medium containing a source of carbon, nitrogen, potassium dihydrophosphate and magnesium sulphate, inoculation of the prepared medium and culturing the basidiomycete. A growth stimulant - arachidonic acid - is also added in amount of 1.0-5.0×10^-5 g/l water. The carbon and nitrogen sources are added to the medium in amount of 10-40 g/l and 6-20 g/l water, respectively, while potassium dihydrophosphate and magnesium sulphate are added in amount of 2.0-2.8 g/l and 0.2-0.3 g/l water, respectively. The culturing process is carried out until maximum pellet formation.

EFFECT: mycelium obtained using said method enables to accelerate formation of fruit bodies and increases yield, and enables to obtain antitumour agents from submerged basidiomycete culture and increase content of lipids in biomass.

8 cl, 1 tbl, 7 ex