METHOD FOR DETERMINING HAPLOTYPE OF MULTIPLE ALLELIC GENES Russian patent published in 2011 - IPC C12Q1/68 

FIELD: medicine.

SUBSTANCE: offered is a method and a set of primers for determining a haplotype of a DNA-target containing two heterozygous polymorphic sites. The method according to the invention provides a) taking a genome DNA sample and amplifying the area on the 3'-side of which there is a first polymorphic site, and on the 5'-side of which the second polymorphic site is found; b) conducting a PCR of said specific area with using one of two primers allele-specific relatively to the first polymorphic site and a downstream primer which is located so that to include the second polymorphic site in an amplicon; c) hybridising the prepared PCR product with two probes one of which is specific to a mutant type sequence, and another one - to a wild type sequence within the second polymorphism and d) determining a haplotype where the first polymorphism is described by a type of an extended upstream primer, and the second polymorphism - by a type of the probe hybridised with the PCR product.

EFFECT: use of the invention provides relatively simple and high precise diagnostic system.

4 cl, 5 dwg, 5 tbl, 1 ex