METHOD OF PRODUCING BACTERIAL LACTOFERRIN Russian patent published in 2011 - IPC C12P21/00 

FIELD: chemistry.

SUBSTANCE: method involves incubation of Klebsiella pneumoniae on a nutrient broth at temperature 37°C to obtain a broth culture, followed by an enzyme immunoassay reaction. Ballast proteins are separated in 2 M ammonium sulphate to obtain a solution. Iron-ammonia alums are then added to the obtained solution to saturate lactoferrin with iron. The iron-saturated solution is then desalinated and buffered with a phosphate buffer with subsequent application onto a column with cationic sephadex G-50/8.5x9 cm/ and elution with discontinuous gradient of sodium chloride concentration is carried out to obtain lactoferrin which is contained in fractions with salt concentration of 0.25 M and 0.3 M.

EFFECT: invention increases output of the desired product.

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