METHOD OF TREATING INJURIES OF TENDON-LIGAMENT STRUCTURES OF ANIMAL EXTREMITIES Russian patent published in 2011 - IPC A61K35/28 A61P19/02 A61P19/04 

FIELD: medicine.

SUBSTANCE: invention relates to field of veterinary. Method includes detection, instrumental examination of injured place and treating impact on the region of injury. In carrying out aspiration of bone marrow from sternum of injured animal, preliminarily sternum localisation is detected by palpation. Local anesthesia is performed and in the course of it trepanopunctures of spongy substance from sternum bone are carried out with medical needle for bone marrow biopsy, provided with mandrin and lateral holes on its end, with 1.5-2 cm step to the depth of penetration into sternum bone up to 1 cm. Bone marrow is sampled in volume 250-500 ml. In realisation of each puncture, aspiration of bone marrow dose is performed with syringe, containing mixture of NaCl 0.9% solution and heparin, it is filtered and all doses of bone marrow are collected into sterile airtight plastic bag, which contains 43 ml of anticoagulant CPDA. After that, bone marrow is separated with isolation of mesenchymal stem cell concentrate by means of apparatus for cell separation Sepax S-100 with volume 20-50 ml. Under control of injured place with ultrasound devices and/or thermography, boundaries of place to be treated and points of cell material introduction are marked with a marker, after which it is injected into the mass of injured ligament or tendon. After introduction of stem cells, injured segment of extremity is immobilised.

EFFECT: method increases efficiency and reduces treatment terms.

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