METHOD FOR EVALUATING OSMOTIC FRAGILITY Russian patent published in 2011 - IPC G01N33/48 

FIELD: medicine.

SUBSTANCE: evaluating osmotic fragility requires 10-fold blood dilution (by 0.02 ml), placing the samples in 5 test tubes with distilled water 0.2 ml containing Ca²⁺ 2.5 mM. They are kept at room temperature for 30, 45, 60, 90 and 120 s, respectively. The haemolysis process is terminated by adding 6% NaCl by 0.2 ml in the test tubes. Further, a microscopy is used to count unhemolysed erythrocytes to derive a water exposure time whereat the erythrocyte count decreases by 50 % from the reference level (T50).

EFFECT: higher accuracy of evaluating the osmotic fragility and more reliable interpretation of research results.

1 dwg, 3 tbl, 1 ex