STRAIN AND BIOSYNTHESIS METHOD OF PRODUCING ANTIBIOTIC MITOMYCIN Russian patent published in 2011 - IPC C12N1/20 C12P1/06 C12R1/465 

FIELD: medicine.

SUBSTANCE: Streptomyces caespitosus 3810/OE strain is a mitomycin C producer prepared from a stock strain recovered from soil by processing with chemical mutagens followed by stage selection, and deposited in the Collection of Cultures of State Institution G.F.Gauze Research Institute for Investigation of New Antibiotics of the Russian Academy of Medical Science, No. INA 01082. For producing mitomycin C, the strain is grown on a sowing medium of the following compoisiton (%): glucose - 0.6-1.4, starch - 0.6-1.4, soya flour - 2-5, corn steep - 0.6-1.0, KH₂PO₄ - 0.01-0.03, chalk - 0.05-0.12, (NH₄)₂SO₄ - 0.06-0.14, main water, pH before sterilisation 6.8-7.2 and then is transferred to an enzyme medium of the following composition (%): starch - 0.6-1.4, sucrose - 1-3, soya flour - 2-4, corn steep - 0.6-1.0, CoCl₂•6H₂O - 0.007-0.012, KH₂PO₄ - 0.02, chalk - 0.05-0.12, (NH₄)₂SO₄ - 0/06-0.04, FeSO₄•7H₂O - 0.01-0.02, MgSO₄•7H₂O - 0.01-0.03, NaCl - 0.2-0.6, main water, pH before sterilisation 6.8-7.2. The strain is grown in an aeration environment for 100-120 h, then mycelium is removed by centrifugation or filtration, and a supernatant containing an end product is extracted by mixed acetonitrile and methyl or ethyl alcohol. A level of strain produced mitomycin C is 85 to 100 mcg/ml.

EFFECT: improved method of producing antibiotic mitomycin.

2 cl, 1 ex