Show metadata Hide metadata

(19)

(11)

2 425 891

(13)

(51)

IPC

C12Q1/68(2006-01-01)

C12N15/31(2006-01-01)

C12R1/01(2006-01-01)

(21) (22)

Application

2010119796/10, 2010-05-17

(24)

Start date

2010-05-17

(22)

Actual filing date

2010-05-17

(45)

Published

2011-08-10

(72)

Inventor

Odinokov Georgij NikolaevichEroshenko Galina AleksandrovnaPavlova Alla IvanovnaAnisimova Ljubov' VladimirovnaKutyrev Vladimir Viktorovich

(73)

Holder

Rossijskaja Federatsija, Ot Imeni Kotoroj Vystupaet Federal'Naja Sluzhba Po Nadzoru V Sfere Zashchity Prav Potrebitelej I Blagopoluchija ChelovekaFederal'Noe Gosudarstvennoe Uchrezhdenie Zdravookhranenija Nauchno-Issledovatel'Skij Protivochumnyj Institut Federal'Noj Sluzhby Po Nadzoru V Sfere Zashchity Prav Potrebitelej I Blagopoluchija Cheloveka

METHOD OF DIFFERENTIATION OF STRAINS OF PRINCIPAL AND NONPRINCIPAL CAUSATIVE PLAGUE AGENT AND PSEUDOTUBERCULOSIS AGENT BY POLYMERASE CHAIN REACTION Russian patent published in 2011 - IPC C12Q1/68 C12N15/31 C12R1/01

Abstract RU 2425891 C1

FIELD: medicine.

SUBSTANCE: method provides recovery of DNA of an analysed strain followed by PCR with using nucleotide primers of terC, ilvN and inv genes of the following sequences: 89-S - AATCAAATCTCGCCCAGC, 89-As -GCTGCGTATCATTTCACC; 45-S - AGTGGTCTGCTTCTCTGG, 45-As -CGGCATACACAGAATACC; inv839 - TACCTGCACTCCCACAAC, inv1007 -CCCATACGCTGATCTACC. The analysed strains are differentiated by matching the sizes of the derived fragments of terC, ilvN and inv genes with the similar fragments in typical strains of principal and nonprincipal plague agents.

EFFECT: invention allows quick, effective and reliable differentiation of the strains.

1 tbl, 3 ex

Similar patents RU2425891C1

Title	Year	Author	Number
METHOD TO DIFFERENTIATE STRAINS OF PLAGUE AGENT OF PRIMARY SUBTYPE OF MEDIEVAL AND ANTIQUE BIOVARS BY METHOD OF POLYMERASE CHAIN REACTION	2012	Odinokov Georgij Nikolaevich Pavlova Alla Ivanovna Eroshenko Galina Aleksandrovna Kutyrev Vladimir Viktorovich	RU2496882C2
METHOD OF SUBSPECIES DIFFERENTIATION OF PLAGUE AGENT STRAINS USING POLYMERASE CHAIN REACTION METHOD	2014	Odinokov Georgij Nikolaevich Nikiforov Konstantin Alekseevich Kukleva Ljubov' Mikhajlovna Krasnov Jaroslav Mikhajlovich Eroshenko Galina Aleksandrovna Kutyrev Vladimir Viktorovich	RU2552611C2
METHOD FOR PLAGUE AGENT STRAINS SUBSPECIES DIFFERENTIATION BY PCR WITH REAL TIME HYBRIDIZATION-FLUORESCENT ACCOUNT OF RESULTS	2016	Nikiforov Konstantin Alekseevich Oglodin Evgenij Gennadevich Kukleva Lyubov Mikhajlovna Nosov Nikita Yurevich Eroshenko Galina Aleksandrovna Kutyrev Vladimir Viktorovich	RU2621869C1
METHOD OF SUBSPECIFIC DIFFERENTIATION OF PLAGUE CAUSATIVE AGENT STRAINS BY SEQUENCING METHOD	2009	Eroshenko Galina Aleksandrovna Kukleva Ljubov' Mikhajlovna Odinokov Georgij Nikolaevich Krasnov Jaroslav Mikhajlovich Guseva Natal'Ja Petrovna Kutyrev Vladimir Viktorovich	RU2404256C1
METHOD OF PLAGUE AND PSEUDOTUBERCULOSIS MICROBE DIFFERENTIATION WITH PARALLEL INTERSPECIES DIFFERENTIATION OF PLAGUE MICROBE STRAINS	2007	Bulgakova Elena Germanovna Gaeva Anna Vjacheslavovna Sukhonosov Il'Ja Jur'Evich Krasnov Jaroslav Mikhajlovich Guseva Natal'Ja Petrovna Kutyrev Vladimir Viktorovich	RU2332464C1
DIFFERENTIATION METHOD OF BIOVARS AND GENOVARIATIONS OF Yersinia pestis STRAINS OF MAIN SUBSPECIES BY MEANS OF POLYMERASE CHAIN REACTION	2014	Nikiforov Konstantin Alekseevich Odinokov Georgij Nikolaevich Oglodin Evgenij Gennad'Evich Kukleva Ljubov' Mikhajlovna Shavina Natal'Ja Jur'Evna Eroshenko Galina Aleksandrovna Kutyrev Vladimir Viktorovich	RU2565554C2
METHOD FOR INDICATION AND IDENTIFICATION OF STRAINS OF THE PLAGUE PATHOGEN BY THEIR BELONGING TO THE SPECIES YERSINIA PESTIS, TO SUBSPECIES, BIOVARS, PHYLOGENETIC BRANCHES AND BY THE PRESENCE OF GENES OF PATHOGENICITY MAIN FACTORS BY THE DNA-CHIP METHOD	2020	Nikiforov Konstantin Alekseevich Utkin Denis Valerevich Kukleva Lyubov Mikhajlovna Eroshenko Galina Aleksandrovna Kutyrev Vladimir Viktorovich	RU2734636C1
METHOD OF IDENTIFICATION AND INTRAGENERIC DIFFERENTIATION OF STRAINS OF SPECIES YERSINIA PESTIS	2009	Trukhachev Aleksej Leonidovich Lebedeva Svetlana Aleksandrovna Vasil'Eva Ekaterina Anatol'Evna Ivanova Vera Stepanovna Rakin Aleksandr Vladimirovich	RU2404251C1
METHOD FOR Yersinia pestis Yersinia pseudotuberculosis STRAIN IDENTIFICATION	2010	Trukhachev Aleksej Leonidovich Arsen'Eva Tat'Jana Evgen'Evna Lebedeva Svetlana Aleksandrovna Alekseeva Ljudmila Pavlovna Vasil'Eva Ekaterina Anatol'Evna	RU2422535C1
METHOD FOR DETECTING ANTIBIOTIC-RESISTANCE GENES IN PLAGUE AGENT STRAINS BY POLYMERASE CHAIN REACTION	2011	Eroshenko Galina Aleksandrovna Odinokov Georgij Nikolaevich Anisimova Ljubov' Vladimirovna Shavina Natal'Ja Jur'Evna Kutyrev Vladimir Viktorovich	RU2456348C1

RU 2 425 891 C1

Authors

Odinokov Georgij Nikolaevich

Eroshenko Galina Aleksandrovna

Pavlova Alla Ivanovna

Anisimova Ljubov' Vladimirovna

Kutyrev Vladimir Viktorovich

Dates

2011-08-10—Published

2010-05-17—Filed