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2 404 256

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IPC

C12Q1/68(2006-01-01)

(21) (22)

Application

2009116913/10, 2009-05-04

(24)

Start date

2009-05-04

(22)

Actual filing date

2009-05-04

(45)

Published

2010-11-20

(72)

Inventor

Eroshenko Galina AleksandrovnaKukleva Ljubov' MikhajlovnaOdinokov Georgij NikolaevichKrasnov Jaroslav MikhajlovichGuseva Natal'Ja PetrovnaKutyrev Vladimir Viktorovich

(73)

Holder

Rossijskaja Federatsija, Ot Imeni Kotoroj Vystupaet Federal'Naja Sluzhba Po Nadzoru V Sfere Zashchity Prav Potrebitelej I Blagopoluchija ChelovekaFederal'Noe Gosudarstvennoe Uchrezhdenie Zdravookhranenija Nauchno-Issledovatel'Skij Protivochumnyj Institut Federal'Noj Sluzhby Po Nadzoru V Sfere Zashchity Prav Potrebitelej I Blagopoluchija Cheloveka

METHOD OF SUBSPECIFIC DIFFERENTIATION OF PLAGUE CAUSATIVE AGENT STRAINS BY SEQUENCING METHOD Russian patent published in 2010 - IPC C12Q1/68

Abstract RU 2404256 C1

FIELD: medicine.

SUBSTANCE: differentiation of strains is carried out by sequencing method. Method includes isolatation of chromosomal DNA is tested strain, carrying out polymerase chain reaction, amplification of fragments of genes rhaS and araC and determination of their nucleotide sequences. Genotype of tested strain is determined by comparison with genotypes of main and not main subspecies.

EFFECT: method allows to carry out subspecific differentiation of strains of plague causative agent quickly, efficiently and reliably.

1 dwg, 2 tbl, 5 ex

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RU 2 404 256 C1

Authors

Eroshenko Galina Aleksandrovna

Kukleva Ljubov' Mikhajlovna

Odinokov Georgij Nikolaevich

Krasnov Jaroslav Mikhajlovich

Guseva Natal'Ja Petrovna

Kutyrev Vladimir Viktorovich

Dates

2010-11-20—Published

2009-05-04—Filed