FIELD: biotechnologies.
SUBSTANCE: determination is performed by the method of polymerase chain reaction (PCR). The method includes extraction of DNA, performance of PCR using synthesised primers Med(24) and Med(19) for amplification of fragments of chromosome genes of the investigated strain. Differentiation is carried out by comparison of dimensions of produced amplified fragments of the investigated strain with dimensions of fragments of genes specific for strains of the medieval biovar 198 and 82 b.p. and for strains of the antique biovar 222 and 101 b.p.
EFFECT: method makes it possible to efficiently and reliably perform differentiation of strains of the main subtype of antique and medieval biovars.
2 ex
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