FIELD: biotechnologies.
SUBSTANCE: method provides for separation of DNA of the test strain, series performance of a polymerase chain reaction (PCR) with Med24, glpD, pCKF primers on a DNA target and with 1.ANT/1.ORI, 2.ANT/2.MED primers of the following composition: Med24-S GTATTTTGTGTCACCCC Med24-As AATGAGACACCGCCAGT glpD-F1 GGCTAGCCGCCTCAACAAAAACAT glpD-R1 GGTCATACAAGAACAAGCCGGTGC pCKF-S TCCGTGCTCATTGGTTCG pCKF-As AGACTTGGCGAACGTGGT 1.ANT/1.ORI-S CGTTCTGCTCTCTGTTTGTC 1.ANT/1.ORI-As GTAGAGATGTGTTGCCCG 2.ANT/2.MED-S AAGACCTTCGCCACCAGA 2.ANT/2.MED-As CCAGGATTCGCCGATTCA. Differentiation is performed by comparison of availability and sizes of the formed amplified fragments of the test strain with dimensions of fragments of typical strains of ancient (genovariations 0.ANT, 1.ANT, 2.ANT), mediaeval (genovariations 2.MED, 2.MED0) and eastern (genovariation 1.ORI) biovars.
EFFECT: invention allows quick and effective separation of strains of plague originator as to their biovar and intrabiovar belonging.
1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR INDICATION AND IDENTIFICATION OF STRAINS OF THE PLAGUE PATHOGEN BY THEIR BELONGING TO THE SPECIES YERSINIA PESTIS, TO SUBSPECIES, BIOVARS, PHYLOGENETIC BRANCHES AND BY THE PRESENCE OF GENES OF PATHOGENICITY MAIN FACTORS BY THE DNA-CHIP METHOD | 2020 |
|
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| METHOD FOR IDENTIFICATION OF YERSINIA PESTIS STRAINS OF A MEDIEVAL BIOVAR WITH SUBSEQUENT DIFFERENTIATION BY PHYLOGENETIC AFFILIATION BY POLYMERASE CHAIN REACTION WITH HYBRIDIZATION-FLUORESCENT ACCOUNT OF RESULTS | 2018 |
|
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| METHOD OF DIFFERENTIATION OF YERSINIA PESTIS STRAINS OF MEDIEVAL BIOVAR ACCORDING TO PHYLOGENETIC AFFILIATION WITH PCR METHOD WITH ELECTROPHORETIC ACCOUNTING OF RESULTS | 2018 |
|
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| METHOD OF DETERMINING THE PHYLOGENETIC AFFILIATION OF STRAINS OF YERSINIA PESTIS OF THE MAIN SUBSPECIES BY ALLELE-SPECIFIC PCR IN REAL TIME | 2022 |
|
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| METHOD OF DIFFERENTIATING TYPICAL AND ATYPICAL STRAINS Yersinia pestis OF MEDIEVAL BIOVAR BY PCR METHOD WITH HYBRIDISATION-FLUORESCENT RECORDING RESULTS | 2014 |
|
RU2550257C2 |
| METHOD OF DETERMINATION OF GENOVARIATIONS OF STRAINS OF PLAGUE AGENT USING MULTILOCUS SEQUENCING METHOD | 2014 |
|
RU2561469C1 |
| METHOD FOR DIFFERENTIATING YERSINIA PESTIS STRAINS OF VARIOUS SUBSPECIES AND BIOVARS BY POLYMERASE CHAIN REACTION AND MULTILOCUS SEQUENCE TYPING | 2011 |
|
RU2471872C1 |
| METHOD TO DIFFERENTIATE STRAINS OF PLAGUE AGENT OF PRIMARY SUBTYPE OF MEDIEVAL AND ANTIQUE BIOVARS BY METHOD OF POLYMERASE CHAIN REACTION | 2012 |
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| METHOD FOR IDENTIFYING YERSINIA PESTIS AND YERSINIA PSEUDOTUBERCULOSIS AND SIMULTANEOUS DIFFERENTIATION OF YERSINIA PESTIS OF MAIN AND CENTRAL ASIAN SUBSPECIES BY MULTIPLEX PCR | 2020 |
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| METHOD FOR PLAGUE AGENT STRAINS SUBSPECIES DIFFERENTIATION BY PCR WITH REAL TIME HYBRIDIZATION-FLUORESCENT ACCOUNT OF RESULTS | 2016 |
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RU2621869C1 |
