FIELD: microbiology.
SUBSTANCE: immobilised biocatalyst contains cells of filamentous fungi, which produce pectinases and are included into matrix of gel carrier containing polyvinyl alcohol. Gel carrier represents cryogel of polyvinyl alcohol with macropores of 0.5-5.0 mcm section. Immobilised biocatalyst is produced on the basis of the following components, taken in the following ratio (wt %): cells of filamentous fungi (by dry mass) - 0.001-0.1, polyvinyl alcohol - 8.4÷12.5; aqueous phase - up to 100. Time of biocatalyst application makes 572-744 parts, average productivity of process by pectolytic activity makes 1.05-1.45 Units.ml-1part-1, and maximum pectolytic activity makes 104160-830000 Units.
EFFECT: invention provides for durable preservation of high mechanical strength by immobilised biocatalyst and vitality of filamentous fungi cells having pectolytic activity.
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Authors
Dates
2010-03-10—Published
2008-07-09—Filed