IMMOBILISED BACTERIA CELLS BIOCATALYST FOR DECOMPOSITION OF METHYLPHOSPHONIC ACID AND ITS ESTERS Russian patent published in 2009 - IPC C12N11/04 

FIELD: medicine.

SUBSTANCE: invention represents a biocatalyst containing immobilised bacteria cells included in polyvinyl alcohol cryogel, used to decompose methylphosphonic acid and its esters. The biocatalyst contains the following components (wt %): bacterial cells biomass - 0.125÷0.725, polyvinyl alcohol cryogel - 7.6÷12.8, aqueous phase - up to 100. Polyvinyl alcohol cryogel is formed in air. As individual bacterial cultures, Pseudomonas species 78G, Escherichia coli BN5α/pTrcTE-OPH, Escherichia coli SG13009 [pREP4]/pTES-His-OPH are used as able to degrade organophosphorous compounds with C-P-bonding. Methylphosphonic acid decomposition rate is 7.3-18 mg/l/h, while its esters are decomposed at 9.6-21.4 mg/l/h throughout 245-310 days.

EFFECT: biocatalyst is characterised with high rate of methylphosphonic acid and its esters decomposition over a long period of time.

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